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DeCS
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Descriptor English:
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Heteroduplex Analysis
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Descriptor Spanish:
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Análisis Heterodúplex
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Descriptor Portuguese:
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Análise Heteroduplex
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Tree Number:
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E05.393.661.250
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Definition English:
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A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions. |
See Related English:
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Base Pair Mismatch
Nucleic Acid Heteroduplexes
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History Note English:
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99
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Allowable Qualifiers English:
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Record Number:
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33874
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Unique Identifier:
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D020180
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Occurrence in VHL:
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Similar:
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DeCS CID-10 SciELO LILACS LIS
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